Title of the article: Investigation of new methods for detectingmicrobes (Batakid comparing two methods, PCR and LAMP)

Background and Aim : In this article, by recalling some new methods of detecting microbes thatare being investigated in immunology, two very suitable and proven new methods that are beingused to detect microbes and viruses have been investigated and researched. One of the advantagesof this research is familiarizing with the types of methods and also focusing on two very importantPCR methods, which are currently providing a great service to society by using them in thediagnosis of the corona virus, and molecular diagnosis by the LAMP method. .Methods : In this article, using the research done by the researchers, descriptive library method isused.Results : In this article, looking at the types of virus detection methods, two new methods, PCRand LAMP, were compared. The LAMP method is used to detect microorganisms because due tothe conditions of low permeability, low temperature and constant temperature, this method causesless damage to the bacteria than the PCR method. It is done at a temperature of ۶۰ to ۶۵ degrees.The sensitivity of the LAMP method is higher than the PCR method in some amounts of DNA.Researchers' research shows that in the LAMP method, it shows about ۱۰ times more sensitivitythan PCR. The PCR method has a much higher price than the LAMP method due to thethermocycler and the need for an equipped laboratory. The LAMP method is faster and cheaper.Considering these differences, the LAMP method can be used to detect a wide range of pathogens.Conclusion : The LAMP method is faster and cheaper. Considering these differences, the LAMPmethod can be used to detect a wide range of pathogens.

کلیدواژه ها:

New methods of detecting microbes ، PCR ، LAMP