The antibacterial activity of bacteriocin-like inhibitorysubstances (BLISs) extracted from clinical Pseudomonas aeruginosastrains

Background and Aim : The emergence of pan-drug resistant strains (PDR) of bacteria has led torenewed efforts to identify alternative agents, such as bacteriocins and bacteriocin-like inhibitorysubstances (BLISs). In recent years, Bacteriocins have been recognized as natural preservatives infood and drug industries, also, nowadays, they are used as substitutes of chemical antibiotics fortreatment of infections.Methods : In a cross-sectional study, ۱۵۰ Pseudomonas aeruginosa strains were isolated fromdifferent Iranian clinical sources (patients with burns, wound and skin infections, UTI, diarrhea,eye infections, human skin wounds, and soft tissue infections) during a ۶-month period. isolateswere investigated by Spot test and Well diffusion test for their ability to produce compoundsexhibiting antibacterial activity. BLISs production was induced by mitomycin C (۲ g/mL) in S.epidermidis.Results : ۲۳ isolated from the P. aeruginosa had potent activity against Staphylococcusepidermidis, Bacillus cereus, Listeria monocytogenes, Klebsiella spp., Staphylococcus aureus,Proteus spp. and Vibrio parahaemolyticus. The BLIS was heat-stable (up to ۱۰۰°C for ۱۵ min) andactive within a pH range of ۳ - ۹.Conclusion : The produced bacteriocin had a wide antibacterial activity spectrum against thegram-positive and negative bacteria, particularly pathogenic bacteria, and was also resistant to heatand pH ranges. As a result, the use of bacteriocin in the food and drug industry, as animal feed,and as a substitute for chemical antibiotics is recommended.

کلیدواژه ها:

BLISs ، Bacteriocin ، Pseudomonas aeruginosa ، Antibiotic ، Drug resistant