Genotyping of extended spectrum beta-lactamases (ESBLs)producing Pseudomonas aeruginosa Isolates from Nosocomial infections

Background and Aim : Background: Pseudomonas aeruginosa (P. aeruginosa) nosocomialinfections is one of the major problems increases mortality and mobility in patients. Objectives:The aim of this research was to determine the molecular epidemiology of ESBLs-producing P.aeruginosa genotypes isolated from nosocomial infections.Methods : One forty-six clinical isolates of Pseudomonas spp. obtained from tertiary referralhospital. Phenotypic identification and PCR using gyrB were performed for P. aeruginosadetection. Extended spectrum beta-lactamases in samples were identified using diskapproximation test and combination disk test (CDT). blaSHV and blaTEM genes were detected byPCR method. Strains were typed with pulse field gel electrophoresis (PFGE), repetitive elementsequence (Rep)-PCR and Enterobacterial repetitive intergenic consensus (ERIC) –PCR methods.Results : A total of ۱۳۴ (۹۱.۷۸%) P. aeruginosa isolated were separated and ۴۱.۷۹% were relatedto nosocomial infection. extended spectrum beta-lactamases analyses test revealed that۵.۹۷% and۶۶.۴۱% isolates harboring blaSHV and blaTEM genes, respectively. Enterobacterial repetitiveintergenic consensus (ERIC) –PCR and Rep-PCR and PFGE showed ۵۶, ۵۵ and ۵۵ differentpatterns, respectively. Pulse field gel electrophoresis indicate that pulsotypes C۳ were dominant.Conclusion : Association between, ESBLs-producing P. aeruginosa, blaSHV and blaTEMpositive P. aeruginosa and ERIC, Rep-PCR and PFGE patterns (p≥۰.۰۵) were not significant.Nosocomial infection, prevalence of ESBLs among the clinical isolates of P. aeruginosa inKurdistan was observed. Periodic review of antibiotic resistance and molecular methods to preventthe spread of infections in hospitals is required.

کلیدواژه ها:

Genotyping Techniques ، beta-lactamase ، Pseudomonas aeruginosa ، Infections ، Hospitals