The immunogenic role of combination of outer membraneproteins Omp۳۴ and BauA against Acinetobacter baumannii infection inmurine mode

Background and Aim : Acinetobacter baumannii is the most important agent of hospital-acquiredinfections worldwide. This bacterium has been regarded as a low-grade but an importantopportunistic pathogen that causes various types of infections, including ventilator-associatedpneumonia, urinary tract infection, skin and wound infections, bacteremia, and meningitis.Recombinant vaccines and specific antibodies are a new treatment strategies for such antibioticresistantinfectious bacteria. However, a small number of bacterial surface antigens were testedthat could only provide partial protection. For this reason, polyvalent (multiple) vaccinescontaining different antigens are needed to provide an acceptable level of protection. This study isoriented on the use of two outer membrane proteins, BauA and Omp۳۴ as a polyvalent vaccine.Methods : Recombinant BauA and Omp۳۴ proteins were expressed, purified, and injected intoBALB/c mice individually and in combination. Both active and passive immunizations werecarried out. The mice were then challenged with a clinical isolate of A.baumannii. Then, the levelof antibody in mice was measured by Indirect ELISA. The animal survival rate was alsodetermined.Results : Elevated antibody production was noted by ELISA in all the immunized groups. Thecombination of BauA and Omp۳۴ proteins rendered good protection compared to the singleadministration of each protein.Conclusion : Polyvalent vaccines that contain different antigens provide an acceptable level ofprotection.

کلیدواژه ها:

Acinetobacter baumunnii . ، Recombinant protein. ، BauA. ، Omp۳۴. ، Immunogenicity. ، Vaccine