Bio-control of Acinetobacter baumannii by Bdellovibrio as apredatory bacteria

Background and Aim : Management of antimicrobial-resistant Acinetobacter baumanni is a greatchallenge for clinical microbiologist. Whereas use the predatory bacteria is the best way to treatinfection diseases caused by antibiotic-resistant bacteria, the aim of this study was to use theautochthonous Bdellovibrio potential to prey Acinetobacter baumannii as a biological controlagent.Methods : To evaluate the effect of autochthonous Bdellovibrio on Acinetobacter baumannii, asa biological control agent, plaque perdition assay, reduction in optical density (OD) and reductionin host cells viability by colony forming unit (CFU) counting in co-cultures after ۰,۲۴, ۴۸ hoursand assay of killing efficiency were carried out.Results : Clear plaques were observed after ۳-۶ days of incubation. In co-cultures, the CFUenumeration of Acinetobacter baumannii was decreased after ۴۸ hours. Also, after ۴۸ hours, ODwas decreased ۰.۷unit. In this research the efficiency ofBdellovibriokilling for Acinetobacterbaumannii was ۸۳%.Conclusion : Base on the results, Bdellovibrio can prey Acinetobacter baumannii as a prey cell.Therefore utilize of Bdellovibrio spp., as biological control agent, for treatment of antimicrobialresistantAcinetobacter baumannii infection suggested in future study

کلیدواژه ها:

Acinetobacter baumannii ، Antimicrobial-resistance ، Bdellovibrio ، biological controlagent.