Antibacterial effects of drug delivery system of mesoporoussilica nanoparticles loaded with rifampin drug on Streptococcuspneumoniae and Pseudomonas aeruginosa

Background and Aim : Bacterial resistance to many available antibiotics is a serious issue inmodern medicine. Streptococcus pneumoniae is a gram-positive, alpha-hemolytic, and oxidasenegativediplococcus and is the main cause of pneumonia that causes other infections such assinusitis, meningitis, and brain abscesses. Pseudomonas aeruginosa is a gram-negative bacillus,which is an opportunistic hospital pathogen, and due to its high resistance to common antibiotics,mortality from its infections is very common. Since the review of the literature shows that the useof nanoparticles is a way to overcome antibiotic resistance, Therefore, the aim of this study was toinvestigate the effects of the drug delivery system containing the antibiotic rifampin, which is abactericidal antibiotic and is used for a variety of bacterial infections, on Streptococcuspneumoniae and Pseudomonas aeruginosa.Methods : Mesoporous silica nanoparticles (MSN) were synthesized using CTAB and TEOS, andusing APTS linker, the amine group was placed on the surface of the nanosphere, and the drugrifampin was loaded in these particles. After checking the properties of the system using DLS,BET, TEM, SEM and checking the amount of drug loading and release, the well diffusion wasperformed on Streptococcus pneumoniae and Pseudomonas aeruginosa bacteria strains.Results : The results of DLS, TEM and SEM showed particles with a size of ۵۰ nm and the BETporosity of these particles was ۳.۴ nm. The loading rate of nanoparticles was ۱۲% and the releaseof rifampin from nanoparticles was found to be influenced by acidic pH. In the evaluation of theantibacterial test using the well diffusion method on Streptococcus pneumoniae and Pseudomonasaeruginosa respectively, free rifampin and nanoparticles loaded with rifampin were used and theresults showed the diameter of the lack of growth from ۹ to ۱۳ mm and from ۵ to ۷ mm,respectively.Conclusion : MSN loaded with rifampin is able to perform its release in acidic pH and is effectivein the treatment of bacterial infections caused by Streptococcus pneumoniae and Pseudomonasaeruginosa.

کلیدواژه ها:

Mesoporous silica nanoparticles ، rifampin ، drug delivery system ، Streptococcuspneumoniae ، Pseudomonas aeruginosa