Molecular Characterization of Antibiotic Resistance Genes inStaphylococcus Isolated from Cell Phone Users’ and Non- Users’ Ears

Background and Aim : Introduction: Resistance to macrolide can be created by erm genes inStaphylococcus. The aim of the current study was to determine whether or not cell phone use canresult in the antibiotic resistance of ۱۶S rDNA, Coa, ermA, ermB and ermC genes in Staphylococciisolated from cell phone users’ and non- users’ ears.Methods : Methods: A total of ۱۵۰ isolates of Staphylococci were tested by the disk diffusionmethod. The isolates were examined by PCR for ۱۶S rDNA, Coa, ermA, ermB and ermC genes.Results : Results: According to PCR results, in two statistical societies, ۶۵.۳۳% cell phone userswith positive Coa had only one erm, ۳۳.۳۳% cell phone nonusers with negative Coa had only oneerm and ۱.۳۴% had a minority of genes, whereas ۲۴% cell phone non-users with positive Coa hadonly one erm , ۴۴% cell phone non-users with negative Coa had only one erm and ۳۲% had aminority of genes. Results showed that ۱۶S rDNA , Coa , ermA, ermB, and ermC genes in the cellphone users group were more prevalent than the other group in Staphylococci isolated from ears.Conclusion : Conclusion: It is revealed that the presence of ۱۶S rDNA, Coa, and erms genes hada significant relation to erythromycin and methicillin. Detection of ermA, ermB and ermC playscrucial roles in the molecular mechanisms, epidemiology of the efflux pump and methylaseerythromycin ribosome. Since antibiotic resistant Staphylococci isolates may mutate and promptconstitutive resistances it is suggested that inducible resistance test should be implemented onerythromycin resistant sensitive isolates to prevent treatment failures.

کلیدواژه ها:

Cell Phone ، Ear ، erm Genes ، Staphylococci