Prevalence of carbapenemase and blaKPC gene in Klebsiellapnumoniae strains isolated from Tabriz hospitals in Iran.

Background and Aim : Background and Aim: Klebsiella pneumoniae is an opportunisticpathogen that is Gram-negative. For the efficient treatment of Klebsiella pneumonia infections,carbapenems are a good option. A class of enzymes called carbapenemases is able to hydrolyzecarbapenems. In order to identify the isolates of Klebsiella pneumoniae that producecarbapenemase in Tabriz, Iran, this study introduced phenotypic and genotypic approaches.Methods : Methods: ۲۳۰ Klebsiella pneumoniae isolates from several Tabriz hospitals were thesubject of this study. The Modified Hodge Test (MHT) was used to detect the isolates of Klebsiellapneumoniae that produce carbapenemase by evaluating the susceptibility of isolates to antibiotics.On an agarose gel, the PCR's final products underwent electrophoresis.Results : Results: For piperacillin and ertapenem, the rates of resistance were observed to be ۸۴%and ۵۰%, respectively. While we did not find MHT positive isolates in cerebral fluids or abdominalcavities, ۶۷% of MHT positive isolates came from urine. Furthermore, the emergency units hadthe fewest cases (۷%) and the most (۷۲%), respectively, of MHT positive isolates in intensive careunits. In none of the isolates was the blaKPC gene discovered.Conclusion : Conclusion: In Tabriz City, Iran, Klebsiella pneumoniae isolates have a relativelylow prevalence of the blaKPC gene.

کلیدواژه ها:

Klebsiella pneumoniae ، Carbapenemase ، PCR method