The prevalence of vanA gene in Methicillin-resistantStaphylococcus Aureus (MRSA) isolated from patients with bedsores

Background and Aim : Introduction: Staphylococcus aureus especially Methicillin-ResistantStaphylococcus aureus (MRSA) is one of the major causes of nosocomial and community-acquiredinfections and nowadays it is known as a health problem in the world due to its resistance to mostantibiotics. This study aimed to determine the prevalence of vanA gene in Methicillin-ResistantStaphylococcus aureus (MRSA) isolated from patients with bedsores.Methods : Materials and Methods: In the current study, ۸۰ clinical isolates were collected frompatiens with bedsores at Loghman-e Hakim hospital, Iranmehr hospital and Tehran wound center.The samples were identified using standard laboratory tests and resistance to cefoxitin disk (۳۰μg)and evaluating the presence of the mecA gene. The resistance of the MRSA isolates to differentantibiotics was checked using the disc diffusion method. The presence vanA gene in these isolateswas investigated using specific primers and polymerase chain reaction (PCR).Results : Results: Of the studied isolates, ۲۹ isolates were identified as methicillin-resistant. Thehighest antibiotic resistance level was observed against erythromycin, and clindamycin; and thelowest was determined against linezolid with ۸۲.۷۵%, ۸۲.۷۵%, and ۱۳.۸% respectively. vanA genewas detected in all of the MRSA isolates. Multidrug resistance (MDR) was observed in almost۷۲.۴۱% of isolates.Conclusion : Discussion and conclusion: The prevalence of vanA gene has increased inStaphylococcus aureus isolates resistant to methicillin and high and multiple isolates ofStaphylococcus aureus resistant to common antibiotics should be considered a serious matter.

کلیدواژه ها:

Methicillin-resistant Staphylococcus aureus ، vanA gene ، nosocomial infections