CRISPR-Like sequences among Helicobacter pylori isolatesand their association with antibiotic resistance

Background and Aim : Part of clustered regularly interspaced short palindromic repeats(CRISPR)-like sequences in antibiotic resistance hasn't been investigated yet. So, this research wasdesigned to study association of CRISPR-like sequences with antibiotic resistance in H. pyloriisolates.Methods : Seventy-two isolates of H. pylori were subjected for study presence of CRISPR likesequences by PCR method and their correction with antibiotic resistance. Antibiotic resistanceprofile of isolates was investigated by microtiter-plate method against tetracycline, andmetronidazole.Results : The CRISPR-like region were detected in ۴۶ (۶۳.۸%) of ۷۲ isolates, respectively. Thefrequency of resistance to tetracycline, and metronidazole in CRISPR-like positive H. pylori were۳۶ (۷۸%), and ۳۶ (۷۸%), respectively.Conclusion : CRISPR-like sequences is involved in antibiotic resistance, so could be served as atherapeutic target and genetic markers of antibiotic resistance

کلیدواژه ها:

Helicobacter pylori ، antibiotic resistance ، CRISPR-like