Investigation of the frequency of resistance to Fluoroquinolonesand the presence of qnrb gene in Klebsiella pneumoniae isolated fromurinary tract infections in Tabriz.

Background and Aim : Background and Aims Klebsiella pneumoniae is one of the importantclinical pathogens and responsible for some nosocomial infections; especially pneumonia,septicemia, and urinary tract infection (UTI). Fluoroquinolones are highly effective antibioticswith many advantageous pharmacokinetic properties including high oral bioavailability, largevolume of distribution, and broad-spectrum antimicrobial activity. With widespread use,antimicrobial resistance to fluoroquinolones has grown Multiple drug resistance among Klebsiellapneumoniae isolates is one of the most important challenges for treating of such infectionsworldwide. This study was conducted with the aim of determining the resistance of Klebsiellapneumoniae isolates collected from Tabriz hospitals against fluoroquinolone antibiotics and thepresence of resistance genes in them.Methods : Methods In this cross-sectional descriptive study, ۲۵۰ gram-negative bacterial isolatesobtained from urinary tract infections in Sina, Madani and Al-Zahra hospitals in Tabriz werestudied. The selective culture media and biochemical test were used for the identification ofKlebsiella pneumonia isolates. Antibacterial susceptibility of isolates was defined to Commonlyused antibiotics in the treatment of infections caused by gram-negative bacteria using diskdiffusion (Kirby – Bauer) method. The presence of resistance qnrb gene was checked by PCRusing specific primersResults : Results ۹۶ isolates of Klebsiella pneumoniae were identified by biochemical tests. Thehighest antibiotic resistance of Klebsiella pneumoniae isolates to ampicillin with ۱۰۰% and thelowest resistance with ۳۰% to chloramphenicol was observed. ۶۸% of the isolates showedresistance to Extended-Spectrum β-Lactamase phenotypically. Resistance to nalidixic acid wasobserved in ۵۰ isolates and to ciprofloxacin in ۴ isolates. qnrb gene was observed in ۵ isolates.Conclusion : Conclusion High resistance to most of the studied antibiotics, especiallyfluoroquinolones, in the studied isolates should be consider as a vital factor and must rigorouslytake into account. Antibiogram and selection of appropriate antibiotic is recommended beforestarting treatment

کلیدواژه ها:

Antibiotic resistance pattern ، Klebsiella pneumonia ، qnrb gene ،