Investigating of resistance to aminoglycosides amongEscherichia coli strains Isolated with class I integrons from surface drinking water supply sources of Ardabil province, Iran

Background and Aim : Aminoglycoside antibiotics resistance is rapidly spreading amongEscherichia coli Antibiotic-resistant bacteria, such as Escherichia coli, being discharged into watersources by humans and animals, in part due to genes carried by integrons. This inquiry aimed Thisinquiry aimed Investigating drug resistance of aminoglycoside antibiotics in Escherichia colistrains with class I integrons in surface drinking water supply sources of Ardabil province.Methods : Escherichia coli strains were isolated and identified applying standard biochemical andmicrobiological techniques from Ardabil province's water supply sources between ۲۰۲۱ and ۲۰۲۲.The class ۱ integron gene was detected using the polymerase chain reaction (PCR). The diskdiffusion method was used to determine antibiotic resistance and sensitivity.Results : The Int I gene was found in ۳۷ out of ۱۲۰ isolates (۳۰.۸%). The antibiotic streptomycinhad the highest resistance (۱۰۰%), whereas gentamycin, tobramycinhad the lowest resistance.Gentamycin and tobramycin on the other hand, had the highest sensitivity of ۱۰۰%, whilestreptomycin had the lowest (۰%). Also, the highest level of the pollution of drinking water supplysources in the province to Escherichia coli was related to Parsabad city (۴۰.۵%) and the lowestlevel of pollution was related to Germi city (۱۳.۵%).Conclusion : According to the findings of this study, the high prevalence of Escherichia colistrains resistant to the antibiotic streptomycin circulating in water sources should be considered amajor concern in terms of antibiotic resistance spreading among bacteria in water.

کلیدواژه ها:

Antibiotic resistance ، Aminoglycoside ، Escherichia coli ، Water sources ، Ardabilprovince ، Class I integrons.