Evaluation of the frequency and quinolone resistance ofCampylobacter spp. in raw milk samples in Alborz province by MAMAPCR method.

Background and Aim : Campylobacteriosis, as an important zoonotic disease, contributes a lotto causing human infectious gastroenteritis, and in addition to causing watery and bloody diarrhea,it also causes secondary diseases such as Guillain-Barre syndrome. Raw milk contaminated withCampylobacter is one of the most common causes of intestinal diseases The effect of consumingcontaminated food. The goals of this study were to identify and separate jejuni and coli species inraw milk samples and to investigate molecular resistance to fluoroquinolones in campylobactersisolated from Alborz province.Methods : ۸۰ samples of cow, sheep and goat raw milk were collected between May and August.The PCR method was optimized to amplify the ۴۰۰ bp fragment of the cadF gene and wasperformed on the sample and ۱۰ samples were declared positive.Then, the MAMA PCR methodwas investigated to identify the resistance of Campylobacter to quinolonesResults : ۸ samples infected with Campylobacter coli species were detected with the formation of۵۰۵ bp fragments, and none of the samples were infected with Campylobacter jejuni species anddid not form ۳۶۸ bp fragments, and the remaining ۲ samples were detected as species other thancoli and jejuni. Number of ۱ sample Of the ۸ positive samples of Campylobacter coli species, therewas a Thr-۸۶ Ile mutation that leads to resistance to fluoroquinolone antibiotics.Conclusion : The results of this study showed the predominance of C. coli species in cases ofCampylobacter contamination of milk samples. The low percentage of resistance tofluoroquinolone drugs in the investigated samples, shows that the use of antibiotics in theinvestigated animal husbandry units was not so widespread as to cause the predominance of drugresistantbacteria