Investigation of Antifungal susceptibility test againstAspergillus flavus species isolated from patients with pulmonaryaspergillosis

Background and Aim : Subject: Aspergillus species are responsible for the highest mortality ratesamong patients with fungal infections. Aspergillus flavus is the common cause of invasiveaspergillosis and pulmonary aspergillosis (PA) in immunocompromised patients. Millions ofpeople worldwide are at risk of aspergillosis each year, especially individuals with impairedimmune function and those with low numbers of neutrophils, solid organ transplant recipients. Thetriazole antifungal drugs, itraconazole, voriconazole, caspofungin, and amphotericin B, arerecommended as first-line drugs in the treatment and prophylaxis of aspergillosis. Azole-resistantAspergillus flavus species have been increasingly identified in the last decade.Methods : Method: This study will be designed to identify and determine the MIC of clinicalisolates of A. flavus according to CLSI M۳۸-A۲ guidelines. Sixty-six Aspergillus flavus isolateswere identified based on the microscopic and macroscopic criteria and molecular identification bysequencing the beta-tubulin (benA) gene.Results : Results: MIC/MEC range for caspofungin was ۰.۰۰۳–۲ μg/ml, compared to ۰.۰۶–۴ μg/mlfor itraconazole, ۰.۲۵–۸ μg/ml for voriconazole, and ۰.۱۲۵–۸ μg/ml for amphotericin B.Aspergillus flavus was the most susceptible to caspofungin (MEC۵۰ = ۰.۰۶ μg/ml) and the mostresistant to amphotericin B (MIC۵۰ = ۲ μg/ml). Resistant to voriconazole, itraconazole,caspofungin, and amphotericin B were reported for ۲, ۱۲, ۶, and ۵ isolates, respectively.Caspofungin exhibited the lowest MEC۵۰/MIC۵۰ (۰.۰۶ mg/mL), followed by itraconazole (۰.۲۵mg/mL), voriconazole (۰.۵ mg/mL), and AMB (۲ mg/mL).Conclusion : Conclusion: This information is necessary to improve patient management and whenan outbreak dealing with drug-resistant infections occurs.

کلیدواژه ها:

Aspergillus flavus ، beta-tubulin ، AFST