RAPD-genotyping of carbapenem-resistant Acinetobacterbaumannii isolated from burn wound infections

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 59

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شناسه ملی سند علمی:

MEDISM23_106

تاریخ نمایه سازی: 16 مهر 1401

چکیده مقاله:

Background and Aim : According to studies, carbapenem-resistant Acinetobacter baumanniistrains are important nosocomial pathogens, especially in burn patients, and classified as an urgentthreat. The aim of current study was to investigate OXA-type carbapenemase genes, and RAPDgenotypingof carbapenem-resistant A. baumannii strains in burn patients.Methods : In this research, ۱۰۶ non-repetitive A. baumannii strains isolated from burn woundinfections were evaluated for susceptibility to antimicrobial agents. Polymerase chain reaction(PCR) was used to detect OXA-type carbapenemase genes and the genetic relatedness among A.baumannii isolates was studied by random amplification of polymorphic DNA (RAPD) typing.Results : All isolates identified as multidrug- and carbapenem- resistant A. baumannii and carriedblaOXA-۵۱-like genes. The blaOXA-۲۳-like genes were the most predominant OXA-typecarbapenemase genes (۹۲.۵%), followed by blaOXA-۲۴/۴۰–like (۸۵.۸%), and blaOXA-۱۴۳-likeand blaOXA-۵۸-like genes were not identified among studied isolates. RAPD- PCR revealed thatthe tested A. baumannii strains are clustered in to ۵ RAPD genotypes with ≥ ۸۰% similarity.Conclusion : The results of the present study indicate a high prevalence of XDR- A. baumanniistrains, which is alarming. In addition, it seems that blaOXA-۲۳-like and blaOXA-۲۴/۴۰–likegenes play an effective role in the development of carbapenem resistance in the studied strains.

نویسندگان

Farzaneh Firoozeh

Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran

Mahnaz Nikibakhsh

Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran

Farzad Badmasti

Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran