Screening and Assessment of Two Potential Bacterial Samplesfor Antimicrobial Activity

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 85

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شناسه ملی سند علمی:

MEDISM23_099

تاریخ نمایه سازی: 16 مهر 1401

چکیده مقاله:

Background and Aim : Antibiotic is a substance produced by microorganisms that inhibits orfully destroys other microorganisms. Since its discovery, diseases caused by bacterial infectionshave significantly reduced and the health of millions of humans around the world has improved.However, emergence of antibiotic-resistance strains is becoming a major concern because soonthe current antimicrobial drugs will lose its effectiveness. There is a need for new antibioticdiscovery as the rate of antibiotic-resistant strains is rising. In this study, tests were carried out toassess the antimicrobial activity of microorganism ۳d-۲ isolated from originally from Broga Hill,Malaysia and sample BCC۲ which was found by accident from a contaminated B. cereus plate.Methods : Initially, cross-streak test method was done to estimate probable antimicrobial activityfrom the mentioned bacterial samples. Kirby-Bauer test was performed using solvent extractsderived from liquid-liquid extraction and antibiotic Rifampicin (۵۰mg/ml) stock, tested againstATCC-approved; Bacillus cereus (ATCC ۱۱۷۷۸), Staphylococcus aureus (ATCC ۲۵۹۲۳),Micrococcus luteus (ATCC ۱۰۲۴۰), E. coli (ATCC ۲۵۹۲۲) and E. coli-Migula (ATCC ۱۱۳۰۳) onMueller Hinton agar. Thin Layer Chromatography (TLC) was performed for extracts from ۳d-۲and BCC۲.Results : Cross-streaking for both samples provided no clear inhibition zones. Kirby-Bauer test’sinhibition zones showed that the extract using diethyl ether, from sample BCC۲ had the strongestresults against Staphylococcus aureus (۱۱mm). TLC approved the largest Rf value was equal to۱.۶۶ and derived from bacteria BCC۲ (mixed with Ethyl ether extract) which basically isconsidered more polar than ones from ۳d-۲, yet extracts from both bacterial strains were certainlypolar. Similarities between Rf values of samples and other common antibiotics show, it is probablethat sample ۳d-۲ and BCC۲ are producing compounds that similar to penicillin and gentamycinrespectively.Conclusion : In conclusion, the antimicrobial compound of both ۳d-۲ and BCC۲ are predicted tobe a narrow-spectrum, belonging to unknown antimicrobial drug category. Further tests andstudies need to be done in future as the identifications are not for certain.

نویسندگان

Bita Rahmani

Department of Biotechnology, Faculty of Applied Sciences, UCSI University, Kuala Lumpur, Malaysia

Andrew Morgan Tennant

Department of Biotechnology, Faculty of Applied Sciences, UCSI University, Kuala Lumpur, Malaysia

Mohammad Reza Shiri-Shahsavar

Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran