The prevalence of flouroqinolon resistance and presence of qnrAand qnrS gens in Escherichia coli isolated from Tabriz, Sina and Alzahrahospitals»

Background and Aim : Abstract Aims and introduction: Escherichia coli is the most importantmember of normal intestinal flora in humans and animals. E. coli is one of the most importantbacterial agent can causing opportunistic infections. Quinolones are synthetic and commonly usedantibiotics for treatment of multiple clinical infections in the world. Quinolones are clinicallyimportant antibiotics, as an ideal component, because of high potency, broad-spectrum activity,good bioavailability and a potentially low incidence of side-effects. Antibiotic resistance toquinolones is increasing in the world. Thus, this study was designed to evaluate the resistance tofluoroquinolone antibiotics and investigate the frequency of plasmid-mediated qnrA, qnrB, andqnrS genes among Escherichia coli isolated of hospitalized patients in Tabriz Alzahra and Sinahospitals, Iran ۲۰۱۹.Methods : Material and method: In the present study, ۱۰۰ E. coli isolates were collected.Antibiotic susceptibility test was carried out by using disc diffusion method. Amplification anddetection of qnrA, qnrB, and qnrS genes were carried out by polymerase chain reaction (PCR)with specific primer.Results : Results: The most effective antibiotic against E. coli isolates was Gentamaycin (۷۸%)but ۹۸% of isolates were resistance to Ampicillin. ۶۲% and ۲۴% of Escherichia coli isolates wereNalidixic acid and Ciprofloxacin-resistant, respectively. qnr genes demonstrated in ۵ isolates. qnrSwere observed in ۴ isolates, and qnrB were identified in one isolates. No qnrA gene was identifiedin this study.Conclusion : Conclusion: According to the results, Because of different antibiotic resistancepatterns in various geographical regions, antimicrobial treatment should be based on localexperience. Therefore, prescribing correct antibiotics can prevent the extension of antibioticresistance through qnr borne bacteria in the future.

کلیدواژه ها:

E. coli ، Fluoroquinolones resistance ، qnrA ، qnrB ، qnrS