Antibacterial Effects of Bacteriocin secreted by Lactobacillusreuteri against pathogenic Helicobacter pylori

Background and Aim : Helicobacter pylori causes gastritis, ulcers and gastric cancer. H. pylorihas infected more than ۵۰% of the population worldwide. Due to the side effects of antibiotics andstrains resistance, the disadvantages of this type of treatment will be greater than its benefits.Reducing H. pylori in the stomach by selective interaction of bacterial cells as an effective methodto compete with stomach pathogens is optimal and it may be effective as a blockage in thetreatment of H. pyloriMethods : In this study, various samples of dairy products in different parts of the country wereused to isolate the L. reuteri strain. Microscopic observation, biochemical and molecular tests andsequencing were performed to confirm the strain. Molecular and biochemical tests were performedto H. pylori identification. Anti-Helicobacter pylori activity of Lactobacillus strains by three cellfreesupernatant (CFS) types was detected. Activity of non-neutralized and non-heat-treated(CFSs۱), non-neutralized and heat-treated (CFSs۲), pH neutralized and non-heat-treated (CFSs۳)against H. pylori strains was evaluated. Inhibition zone test for H. pylori was performed using L.reuteri strains. lactic acid produced by L. reuteri was neutralized.Results : An agar-well diffusion method was used to assess CFS effect on H. pylori growth. Theinhibition zones of high concentrations of CFSs were more obvious, indicating that CFS had thepotential ability to inhibit H. pylori growth. According to results, the agar-well diffusion testshowed significant inhibitory effects of L. reuteri against H. Pylori. Formation of inhibition zonewas significantly due to presence of antimicrobial metabolites (e.g. bacteriocin) not secretive acidlactic.Conclusion : In summary, based on these results it can be found that the L. reuteri which is aprobiotic can be applied in therapeutic application for treatment of disease related to H. pylori ingastrointestinal tract. Bacteriocin-like inhibitory substance activity can be an advantage for theprobiotic choice for H. pylori infection control. If bacteriocin produced by L. reuteri hasantimicrobial properties against Helicobacter pylori, it can be considered as a new generation ofprobiotics, postbiotics.

کلیدواژه ها:

L. reuteri ، H. pylori ، bacteriocin ، agar-well diffusion ، postbiotic