CORROBORATE A NEW SURROGATE VIRUSNEUTRALIZATION TEST FOR THE DIAGNOSTIC PURPOSES OFHUMORAL IMMUNITY AGAINST SARS-COV-۲

Background and Aim : The humoral response has a crucial role in immunity against SARS-CoV-۲, which is elevated by infection and vaccination. Currently, several technics have been establishedto evaluate the neutralizing antibodies (NAbs) levels in the blood serum. In this manner, antibodiesagainst Spike glycoprotein of SARS-CoV-۲ are the most important targets. The aims of this studywere humoral immunity assessment in individuals with different backgrounds and validation of anew surrogate virus neutralization test against SARS-COV-۲.Methods : We compared the conventional virus neutralization test (cVNT) as a gold standard andanti-S Electrochemiluminescence immunoassay (eCLIA) (Roche Diagnostics, Switzerland) witha surrogate virus neutralization test (sVNT) (iFlash-۲۰۱۹-nCoV Nab assay, YHLO, China) invaccinated, recovered and hybrid immunized population.Results : Our finding demonstrates that a reduction in humoral immunity against SARS-CoV-۲may occur over time. By The cVNT titers ? ۱:۱۶, ۷۴.۵ U/ml and ۴۹.۴ IU/ml for anti-S eCLIA andsVNT represent the semi-equal thresholds respectively.Conclusion : According to our observations, increasing the herd immunity against SARS-CoV-۲by vaccination is critical to be taken into account. Furthermore, we suggest that sVNT could be areliable routine serologic assay that performs high specificity, without cross reactivity.

کلیدواژه ها:

SARS-CoV-۲ ، neutralizing antibodies ، sVNT ، eCLIA ، cVNT