Use of specific Lytic nano-phage (combination ofbacteriophage and nanotechnology) in the treatment of drug-resistanttuberculosis in vitro

Background and Aim : The emergence of multidrug-resistant tuberculosis (MDR), which meanstuberculosis is caused by an isolate of Mycobacterium tuberculosis that is at least resistant toisoniazid and rifampin, is of global importance. Unlike antibiotics, bacteriophages act specificallyand do not disturb the natural balance of microflora. Therefore, they are present at the place ofinfection where their existence is more necessary and they destroy their host, the bacterium thatcauses the infection, in a precise way. The purpose of this research is to investigate the efficiencyof isolated specific bacteriophage against drug-resistant Mycobacterium tuberculosis bacteria andits combination with SLN solid lipid nanoparticles as nanocarriers in laboratory conditions.Methods : In order, isolation of Mycobacterium tuberculosis from clinical samples, primaryisolation of bacteriophage, purification of bacteriophage, determination of bacteriophagemorphology, determination of host and specificity of bacteriophage, test to determine thesensitivity of isolated bacteriophage to temperature and pH, preparation of respiratory spray(MDIS) containing specific lytic bacteriophage Using solid lipid nanoparticles (SLN),Mycobacterium tuberculosis bacterium was investigated in terms of invasiveness and the effect ofits specific bacteriophage on the specific cell line.Results : Bacteriophage isolated with different concentrations has shown a good ability to reducethe number and eliminate Mycobacterium tuberculosis bacteria. The specific bacteriophageplaques isolated against the studied bacteria were specific and against other pathogenic bacteria,they have no lytic function.Conclusion : This study has shown that bacteriophage or a combination of phage can reduce orcompletely eliminate Mycobacterium tuberculosis bacteria in laboratory conditions. These resultsshowed that the treatment by bacteriophages has the potential to be used as an alternative proposedmethod to prevent the use of antibiotics in order to reduce or eliminate Mycobacteriumtuberculosis infection. Therefore, they can be used as biologically capable tools against bacteria inpractical conditions.

کلیدواژه ها:

Bacteriophage ، M. tuberculosis ، Drug resistance ، Nanotechnology