Isolation of lytic bacteriophage ECP-ST against AntibioticResistant Escherichia coli strains isolated from urinary tract infection

Background and Aim : Uropathogenic E. coli (UPEC) is a Gram-negative bacterium and thecause of a wide range of (UTI) urinary tract infections. This bacterium is resistant to manyantimicrobial agents and has become a major problem for societies. Therefore, new methods ofcontrolling infections resistant to antibiotics are much needed. Meanwhile, bacteriophages can beused as a suitable alternative treatment, which are less resistant to them and also have few sideeffects. In this regard, the aim of the present study is to isolate lytic bacteriophage against severalresistant strains of E. coli isolated from urinary tract infections.Methods : In this research, in order to collect resistant E. coli bacteria strains, which are the causeof urinary tract infection, different samples of E. coli isolated in Isfahan medical diagnosislaboratories were collected. Then biochemical diagnostic tests as well as disk diffusionantibiogram test were performed for them to check antibiotic resistance. Also, sewage and riverwater samples were used for the isolation of specific bacteriophages and were examined by twolayeragar method and spot tests and overlay tests.Results : In this research, we used bacteria that were resistant to cefepime, ceftazidime, imipenem,and ciprofloxacin, and finally we obtained bacteriophage ECP-ST. This bacteriophage had lyticproperties against eight bacterial strains out of twenty five strains collected. And it has transparentplaques with a diameter of ۲ to ۳ mm, and their image was observed under the electron microscope,which shows that the ECP-ST phage has a symmetrical series and a retractable tail.Conclusion : According to the results of this research, ECP-ST phage can be a suitable option forthe preparation of phage mixture against UPEC strains for the treatment of urinary tract infections.

کلیدواژه ها:

Bacteriophage ، uropathogenic E. coli ، Urinary Tract Infection ، Multi DrugResistance