Changes in the microbial genomic pattern of the blood ofpatients with Crohn's disease in the flare and remission phases

Background and Aim : Crohn's disease (CD) has a chronic course, which environmental, geneticand bacterial flora are the main factors causing this condition. There is a lack of data about the roleof bacteria in Crohn’s disease incidence and its relation with gradual changes of bacterialbiomarkers in blood of CD patients.Methods : Nineteen CD patients were enrolled in this study. CD activity scores, biological, clinicaland demographic data of the patients were recorded during ۳-months follow up. Aerobic andanaerobic bacteriological culture was done on blood samples and ۱۶SrRNA sequencing were doneon extracted DNA from PBMCs.Results : Bacteriological culture showed bacteriemia with Streptococcus spp. and Clostridiumspp. in two active CD patients. Bacterial population in flare phase of active patients weresignificantly higher than their remission phase. This difference was mostly associated with thedecrease in the population of gram-negative bacteria such as salmonella, sphingomonas,bradyrhizobium and gram-positive bacteria such as streptococcus, enterococcus and lactobacillusin remission phase.Conclusion : Bacterial diversity was decreased in remission phase of CD patients. The quantityand diversity of bacterial genera were involved in severity of CD patients.

کلیدواژه ها:

Crohn’s disease ، Gene expression ، ۱۶SrRNA sequencing ، Bacteremia