Evaluation of immunogenic efficacy of Hma.FdeC.UpaB.CTBrecombinant protein in associated with chitosan against UropathogenicEscherichia coli

Background and Aim : Urinary tract infections (UTIs)are the most common bacterial infectionsand usually caused by Uropathogenic Escherichia coli (UPEC). Serious challenges such as chronicrecurrent UTIs, the need for long-term prophylactic antibiotic therapy in the antibiotic resistanceera highlights the necessities for effective preventive methods, such as vaccination against UTIs.Methods : FdeC, Hma, UpaB and also Ctb (as build-in adjuvant) were chosen to design a multiepitopesubunit vaccine containing Bcell linear epitopes using different bioinformatic methods.Recombinant protein expression was performed using the BL۲۱(DE۳)/pET۲۸ expression systemand purified through a Ni-NTA column. The designed proteins were encapsulated in chitosannanoparticles. Female ۶–۸ weeks old BALB/c mice were immunized intranasally with vaccineprotein or chitosan-encapsulated vaccine protein. Total IgG, IgG isotypes (IgG۱, IgG۲a) and IgMresponses of immunized mice were measured by ELISA to determine antibody responses.Results : Expression of the vaccine protein was confirmed by SDS- PAGE and western blot. Theanalysis of antibody assay shows that vaccine protein in associated with chitosan significantlystimulates humoral immunity.Conclusion : Our results showed that intranasal administration of the designed vaccine formulatedwith chitosan has the potential to stimulate the protective humoral immune response againstUPEC.

کلیدواژه ها:

Urinary tract infections ، multi-epitope subunit vaccine ، chitosan