A survey on ESBL, MBL Enzymes, and AcrAB-TolC EffluxPump Frequency Correlation with MDR in Clinical Enterobacter spp.
محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 115
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شناسه ملی سند علمی:
MEDISM23_058
تاریخ نمایه سازی: 16 مهر 1401
چکیده مقاله:
Background and Aim : Enterobacter cloaca and Enterobacter aerogenes are opportunisticpathogens that cause nosocomial infections. Multidrug resistance, in particular, the presence ofExtended-spectrum beta-lactamase enzymes, Metallo-beta-lactamases, and efflux pumps haveposed major problems in treatment. The aim of this study was to evaluation of ESBL, MBLenzymes, and AcrAB-TolC efflux pump in Enterobacter isolated from hospitals of Hamadan andTehran.Methods : A total of ۴۲ isolates were identified as Enterobacter species and were evaluated forantibiotic susceptibility using the disk agar diffusion method, the MIC of cefotaxime was usingthe E-test strip. PCR method was used to identify ESBL, MBL beta-lactamase, and AcrAB-TolCefflux pump genes, using the Double-Disk Synergy Test and Imipenem-EDTA combined disc.Statistical analysis was performed using SPSS software (Version ۲۴).Results : Ceftazidime had the highest percentage of antibiotic resistance (۷۱.۵%), while TGC hadthe lowest resistance to antibiotics (۴.۶%). ۷۶% of the isolates were resistant to cefotaxime (MICtest) and ۷۴.۶% of the isolates were MDR. In phenotypic analyses, ۸۸.۵% of isolates were ESBLpositive and ۶% were MBL positive. Furthermore, PCR test results for ESBL genes revealed thatthe largest presence of genes was connected to TEM (۹۶%), SHV (۶۶%), and CTX-M (۶۸%),which were among six separate gene groups. CTX-M genes CTX-M۱ (۷۲%) and CTX-M۱۵ (۶۸%)were discovered in the isolates. Furthermore, the frequency of AcrAB-TolC efflux pump geneswas Acr-A, Tol-C, and Acr-B, ۲۸%, ۱۸% and ۱۲%, respectively.Conclusion : The high incidence of Enterobacter strains to produce ESBLs with the TEM, SHV,and CTX-M genes found in this study has been identified as a severe concern in hospitals, posingserious issues for infection management and antibiotic therapy. On the other hand, there was acorrelation between the existence of the IMP Metallo-beta-lactamase genes and the developmentof antibiotic resistance, also the presence of the AcrAB-TolC efflux pump genes. Colistin,amikacin, tigecycline, and imipenem have a low resistance to isolates and can be used to treatinfections.
کلیدواژه ها:
نویسندگان
Mohammad Taheri
Department of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Shabnam Khanialiakbari
Department of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Rasoul Yousefimashouf
Department of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran