The effect of ampicillin/sulbactam-colistin-eluting noisomenanoparticles against the expression of biofilm-associated genes (pgaA,pglL) of Acinetobacter baumannii isolates

Background and Aim : Acinetobacter baumannii is an emerging cause of nosocomial infections.The isolation of strains resistant to multiple antibiotics is increasing at alarming rates. Thedemonstrated ability of nosocomial strains to grow as the biofilm is believed to play a significantrole in their persistence and antibiotic resistance. This study aimed to evaluate the effect ofampicillin /sulbactam-colistin eluting noisome nanoparticles against the expression of biofilmassociatedgenes of Acinetobacter baumannii isolates.Methods : In this study, among۴۰ clinical samples, ۱۰ high biofilm-forming isolates weredetected. Niosomes were prepared using the thin-film hydration method and were characterizedby zeta potential measurement and Field Emission Electron Microscope (FE-SEM). The ampicillin/sulbactam-colistin entrapment efficiency was determined. The expression levels of biofilmassociatedgenes(pgaA, pglL) were quantified by the Quantitative Real-Time PCR method.Statistical analysis was performed using one-way ANOVA test.Results : The round-shaped ampicillin /sulbactam-colistin eluting noisomes were ۲۳۰ nm and had-۲۵mV zeta potential. The encapsulation of ampicillin /sulbactam and colistin in the niosomes was۷۸% and ۸۹%, respectively. Noisomes containing ampicillin /sulbactam-colistin couldsignificantly reduce the expression of pgaA and pglL (p<۰.۰۵).Conclusion : The results indicated that ampicillin/sulbactam-colistin- loaded niosomes reducedthe expression of genes related to biofilm formation and the antibioflim activity ofampicillin/sulbactam-colistin- eluting niosomes was confirmed.

کلیدواژه ها:

Acinetobacter baumannii ، biofilm-associated genes ، niosome nanoparticle