Enhanced anti-biofilm activity of the minocycline-and-galliumnitrate-containing niosomes against Acinetobacter baumannii in themouse pneumonia model

Background and Aim : Acinetobacter baumannii is a worldwide health issue due to its highantibiotic resistance and ability to construct biofilms. Nanoparticles (NPs) with highbiocompatibility, high penetrating power and low medication dose can successfully treatantibiotic-resistant infections. This study will use a mouse pneumonia model to evaluate the antibiofilmeffectiveness of niosomes containing minocycline and gallium nitrate (GaN).Methods : This study's clinical sample was isolated among diverse bacterial biofilms developedon the lungs of patients hospitalized in Logman hospital, Tehran, Iran. The biofilm of A. baumanniiwas considered the most lethal strain to be evaluated. To increase their anti-biofilm characteristics,Minocycline and GaN were encapsulated in niosomes as biocompatible drug carriers. Theniosomes' size, zeta potential, shape, stability, drug entrapment efficacy, drug release pattern, MIC,and MBC were studied. It was generated by giving A. baumannii suspension intranasally toanesthetized mice whose immune systems had already been compromised twice bycyclophosphamide. The infection was proven before the mice were treated using PCR. Aftertreatment, the lungs were excised sterile and stained with (Hematoxylin and eosin) H&E todetermine histologic symptoms, inflammation, and intercellular secretions. The drugs' cytotoxiceffects were assessed in the liver and spleenResults : The niosomes investigated contained minocycline, and GaN had an average size and zetapotential of ۲۳۰ nm and -۴۰ mV. Niosomal formulations feature a high rate of drug entrapmentand a delayed drug release rate. Niosomes containing minocycline and GaN eradicated biofilmsafter ۱, ۳, and ۵ days. The mice given the combination of the two compounds required less time tobe treated than the animals given the single medication (minocycline).Conclusion : The therapy group survived longer than the control group. The minocycline andGaN-loaded niosomes could be considered promising candidates for treating the infections causedby A. baumannii and biofilm formation

کلیدواژه ها:

Niosome ، Minocycline ، Gallium Nitrate ، Pneumonia Model ، Acinetobacterbaumannii ،