MICROBIOME ANALYTICS IN THE ۲۱ST CENTURY:PERSPECTIVES AND CAVEATS
محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 55
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شناسه ملی سند علمی:
MEDISM23_013
تاریخ نمایه سازی: 16 مهر 1401
چکیده مقاله:
Background and Aim : Humans exist as meta-organisms comprised of both the macroscopic hostand its symbiotic commensal microbiota. With approximately ۱۰۰ trillion cells, bacteriaoutnumber host cells by at least a factor of ۱۰ and express at least ۱۰۰-fold more unique genesthan their host’s genome. The tremendous enzymatic capability of the microbiome results in aplethora of metabolites found in humans which play a fundamental role in nearly all aspects ofhost physiology and disease development including metabolic, cardiovascular and even neuropsychatricillnesses. Since ۲۰۰۰, large-scale ۱۶S rRNA or metagenomic studies have dramaticallyexpanded the knowledge about diversity of the human gut microbiome. Approximately ۸۰% of thebacteria found by molecular tools are uncultured so far, and hence can be characterized only byhigh throughput sequencing and adequate bioinformatics analysis. Applying our patented NGSqualitycontrol tools we completed four European external quality assessments (EQAS) comparingresults from different next generation sequencing (NGS) centers with special emphasis on criticalpreanalytic steps, nucleic acid preparation and bioinformatic data processing. Furthermore, ourgoal is to achieve a functional understanding of bidirectional microbe-host interactions in healthand diseases (e.g. Graft versus host disease and depression), beyond largely descriptivecompositional and metagenomic analyses.Methods : not applicableResults : not applicableConclusion : comprehensive survey
کلیدواژه ها:
نویسندگان
André Gessner
Institute of Medical Microbiology and Hygiene, University of Regensburg, Germany