Investigation of associated between coronary artery disease risk and mir-۱۴۶a (rs۲۹۱۰۱۴۶) polymorphism in an Iranian population

Backgrounds: Coronary artery disease (CAD) is the leading cause of death in humansworldwide. CAD is considered to be caused by both environmental and genetic factors as well asthe interaction of these two factors. Apart from modifiable risk factors such as diet and tobaccouse, genetic factors have been estimated to account for ۴۰–۶۰% of the risk for CAD inepidemiology, family, and twin studies. Hence genetic risk factors predisposing people to CADremain largely unknown. MicroRNAs (miRNAs) are a class of small, non-coding RNAmolecules with ۲۱–۲۳ nucleotides in length, and negatively regulate gene expression at the posttranscriptionallevel through translational repression or mRNA degradation. There are more thana thousand miRNAs reported in a human genome. There is an increasing body of evidence thatmiRNAs play a critical role in the control of key biological processes including development,differentiation, growth, and metabolism as well as pathophysiology of neurodegenerativedisease, cancer and cardiovascular disease. This study investigated the influence of the miR-۱۴۶aGC rs۲۹۱۰۱۶۴ in Iranian population with CAD.Materials and Methods: One hundred and twenty angiografically confirmed CAD patients, and۱۱۸ healthy controls with no symptoms of CAD. Genomic DNA extraction from whole bloodwas performed for all of the participants. Genotyping of the polymorphism was done bypolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Results: Frequencies of CC, GC and GG genotypes were ۰ %, ۳۸.۱%, ۶۱.۹% in the controlgroup and ۳.۳%, ۳۸.۳% and ۵۸.۳% in CAD patients respectively. There was no significantdifference in frequencies of the genotypes between case and control groups (P = ۰. ۰.۱۸۵۷).Conclusion: The sample size was relatively small. A future study with larger sample size isneeded.

کلیدواژه ها:

Coronary artery disease ، Mir-۱۴۶a ، Polymorphism