Investigation of LincFOXF۱ expression in colorectal cance\

Backgrounds: Colorectal cancer (CRC) is the third most common cancer in the world. Longintergenic noncoding RNAs (LncRNAs) are of paramount importance in the underlyingmolecular mechanisms of CRC initiation and progression. According to the recent bioinformaticsstudies, lincFOXF۱ may be involved in the CRC progression and metastasis via induction ofepithelial-mesenchymal transition (EMT). For this reason, the aim of this study was investigationof lincFOXF۱ expression in CRC tissue samples and evaluation of its potential as diagnosticbiomarker in this cancer for first time.Materials and Methods: The colorectal tumor samples and adjacent normal tissue samples werecollected from thirty patients. After total RNA extraction from samples and cDNA synthesis,Real-time PCR was used to measure the LincFOXF۱ expression. Paired t-test was used forcomparison of LincFOXF۱ expression in tumor and normal samples and receiver operating curve(ROC) was used to assess the diagnostic value of LincFOXF۱.Results: The qPCR results showed that the expression of LincFOXF۱ was down-regulated ۳/۷۶times in thirty paired colorectal cancer specimens (p-value = ۰.۰۴۲۳). The AUC of ROC curvewas ۰.۶۸۸۳ and revealed that the expression level of LincFOXF۱ can detect up to ۶۸.۸۳% ofcases of colorectal cancer and can be used as a diagnostic biomarker.Conclusion: Finally, the authors suggest that the considerably down-regulation of LincFOXF۱in CRC tumor samples and its potential as biomarker highlight the need to further investigationof this lncRNA in larger population and cohort studies.

کلیدواژه ها:

LincFOXF۱ ، CRC ، Cancer progression ، Cancer metastasis ، LncRNA