The significant expression of P۴۵۰ drug metabolism pathway between untreated tumors and normal-adjacent tissues in breast cancer patients

Backgrounds: The cytochrome P۴۵۰ (CYP) family is a group of enzymes that contribute to thecell proliferation, progression and drug metabolism of breast cancer (BC). This pathway isaffected in the metabolism of pro-carcinogens and response to the treatment as well, so their roleremains controversial. Nowadays, with the advent of bioinformatics analysis and highthroughput-based methods, it is possible to study and describe the expression profile of tumorsand determine the genes' functions and their pathways toward diagnosis, prognosis andtherapeutic targets. Our study aims to detect the significant genes and pathways involvedbetween cancerous and non-cancerous breast tissue by analysis of microarray datasets.Materials and Methods: Based on our inclusion and exclusion criteria, six microarray studieswere obtained from Gene Expression Omnibus (GEO). Finally, four datasets “GSE۱۳۹۰۳۸,GSE۲۲۳۸۴, GSE۳۱۵۸۹ and GSE۲۴۱۲۴” were chosen among them. We analyzed raw data with“R” software, “limma” and “AgiMicroRna” packages, then normalized by “SVA” package. Atthe next step, Gene Set Enrichment Analysis (GSEA) was performed to determine the keypathways.Results: The result based on GSEA was performed between untreated tumor and adjacentnormalsamples and finally P۴۵۰ drug metabolism circumscribed through Kyoto Encyclopedia ofGenes and Genomes (KEGG) database. Finally, ۲۸ top genes were determined to confirm thevital role of P۴۵۰ pathway among downregulated ones in cancer.Conclusion: This study manifested the reduction expression of P۴۵۰ Drug Metabolism pathwayin BC and its association as biomarkers in this malignancy.

کلیدواژه ها:

Bioinformatics analysis ، GEO ، GSEA ، Breast cancer ، P۴۵۰ Cytochrome Pathway