Study of BDNF gene expression in Alzheiemerʹs patients

Background: Alzheimer’s disease is one of the most destructive neurodegenerative disases. It ismostly seen in people over ۶۵ years old, and is associated with the loss of neurons in specificareas of the brain. Brain –derived neurotrophic factor(BDNF) is involved in the development ofneural survival and the synaptic process of memory. BDNF level vary according to the severityof the disease.BDNF can be involved as an important biomarker in the diagnosis of Alzheimerʹsdisease.we can suggest a treatment for alzheimerʹs disease by adjusting the amount BDNF andsuppressing the amyloid beta peptide.Materials and Methods: RNA was isolated from blood of sick and healthy individuals usingTRIZOL reagent. cDNA synthesis was done by anchored olig-dTs and RT-PCR was carried outby using BDNF specific primers. We used GAPDH gene as internal control gene. Then qRTPCRperformed to assess of BDNF gene expression level.Results: Our results indicated that there is a significant relationship between the levels ofexpression of BDNF with alzhemierʹs disease. Real-time PCR results showed that BDNF geneexpression is significantly increased in the early stages of the disease and is almost two foldhigher than healthy subjects.Conclusion: Overall, we found that BDNF circulating amount can be used as an importantbiomarker to diagnosis of Alzheimerʹs disease. However, future additional researches arenecessary to confirmation of these results.

کلیدواژه ها:

BDNF ، Alzheimer ، biomarker ، gene expression