Potential biomarkers and signaling pathways involved in helicobacter pylori-induced gastric cancer: A network-biology approach

Backgrounds: Gastric cancer (GC) is the second most frequent malignancy-related deathworldwide. The ۵-year survival rate of patients remains fragile. There is a requirement todiscover biomarkers for prognosis approaches. Chronic infection with Helicobacter pylori (H.Pylori) is closely associated with the progression of GC. We aimed to identify the genesassociated with poor/favorable prognosis in H. Pylori-induced GC.Materials and Methods: The tissue miRNA dataset GSE۵۴۳۹۷ was obtained from the GEOdatabase and analyzed to identify differentially expressed miRNAs (DEMs) in patients with H.Pylori-induced GC compared to H. Pylori-positive patients with non-cancerous tissue. A proteinproteininteraction (PPI) network was built and analyzed. The biological processes (BPs) andpathways associated with genes involved in the main clusters were studied. The hub genes in thePPI network were identified, and the survival analysis was performed.Results: A total of five DEMs were found with the criteria of P < ۰.۰۱ and the absolute value ofLog۲ fold change > ۱. The most significant pathways and BPs affected in patients with H.Pylori-induced GC were primarily associated with the ubiquitination, neddylation, and ciliaryprocess. Survival analysis illustrated that the overexpression of DOCK۴, GNAS, CTGF, TGFb۱,ESR۱, SELE, TIMP۳, SMARCE۱, and TXNIP was associated with poor prognosis, whileincreased expression of MRPS۵ was associated with favorable prognosis in GC patients.Conclusion: DOCK۴, GNAS, CTGF, TGF-b۱, ESR۱, SELE, TIMP۳, SMARCE۱, TXNIP, andMRPS۵ may be considered as prognostic biomarkers for H. Pylori-induced GC. However,confirmation is required in the future.

کلیدواژه ها:

Biomarkers ، Helicobacter. Pylori ، Prognosis ، Protein interaction maps ، Stomach neoplasms ، Survival analysis