Investigation of LincNRAV expression in colorectal cancer

Backgrounds: Colorectal cancer (CRC) is the fourth most common but silent cancer in Iran.Long noncoding RNAs (LincRNAs) involve in the tumor formation, angiogenesis, proliferation,migration, apoptosis and differentiation. Previous studies on role of LincNRAV in severalcancers and respiratory diseases showed LincNRAV effects on Rab۵c, miR-۵۰۹-P۳, andMAP۳K۸, directly and indirectly. These genes involve in CRC progression and chemotherapyresistant. Based on these evidences, the aim of this study was investigation of LincNRAVexpression in CRC tissue samples and evaluation of its potential as diagnostic biomarker in thiscancer for first time.Materials and Methods: The colorectal tumor samples and adjacent normal tissue samples werecollected from thirty patients. After total RNA extraction from samples and cDNA synthesis,Real-time PCR was used to measure the LincNRAV expression.Results: The qPCR results showed that the expression of LincNRAV was down-regulated incolorectal cancer specimens compared to normal tissue samples (p-value = ۰.۰۰۳۸). The AUC ofROC curve was ۰.۶۷ and revealed that the expression level of LincNRAV can detect up to ۶۷%of cases of colorectal cancer.Conclusion: The considerably down-regulation of LincNRAV in CRC tumor samples and itspotential as biomarker highlight the need to further investigation of this LncRNA in largerpopulation and cohort studies.

کلیدواژه ها:

Colorectal cancer ، Linc RNA NRAV ، Rab۵c ، miR-۵۰۹-P۳ ، MAP۳K۸