Investigation of Lnc DA۱۲۵۹۴۲ expression in colorectal cancer

Backgrounds: Colorectal cancer (CRC) accounts for ۱۰% of global cancer incidence and ۹.۴%of cancer mortalities. LncRNAs involved in the tumor formation, angiogenesis, proliferation,migration, and etc. Bioinformatics studies showed that LncDA۱۲۵۹۴۲ may be involved in theprogression and metastasis of CRC. On the other hand, co-expression of LncDA۱۲۵۹۴ andvitamin D receptor (VDR) has been demonstrated in the breast cancer. Vitamin D deficiency isassociated with high rate of CRC incidence and mortality. The aim of this study wasinvestigation of LncDA۱۲۵۹۴۲ expression in CRC tissue samples.Materials and Methods: The colorectal tumor samples and adjacent normal tissue samples werecollected from thirty patients. After total RNA extraction from samples and cDNA synthesis,Real-time PCR was used to measure the LncDA۱۲۵۹۴ expression. Paired t-test was used forcomparison of LncDA۱۲۵۹۴ expression in tumor and normal samples.Results: The results of qPCR showed that there is no statistically significant difference in theexpression level of DA۱۲۵۹۴۲ between normal and tumor samples (p-value ۰.۸۲۹۲). Expressionof this Lnc in the metastatic patients and patients with higher stages of CRC is lower rather thanother patients (p-value ۰.۰۰۵۲).Conclusion: Lnc DA۱۲۵۹۴۲ may acts as a tumor suppressor in the CRC and decrease ofexpression has important role in CRC progression and metastasis. This is suggested that thepotential of this LncRNA as a biomarker in the different stages of CRC is evaluated in the largepopulations.

کلیدواژه ها:

LncDA۱۲۵۹۴۲ ، Colorectal cancer ، Vitamin D receptor ، Cancer progression ، Cancer metastasis