rs۱۰۷۱۹ polymorphism in DROSHA gene may destroy binding site of has-miR-۶۶۴a-۳p and has-miR-۱۲۹۸-۵p

Backgrounds: Breast cancer is the most common cancer for women worldwide and is mainfactor of cancer mortality. Single nucleotide polymorphisms (SNPs) in microRNA biosynthesispathways may result in the less or gain of microRNA function that can act as an oncogenic ortumor-suppressive. Dysregulation of microRNA biogenesis pathway genes is involved in theinitiation and progression of several human cancers including breast cancer. One of the mostimportant genes in this field is DROSHA. Previous studies have shown that rs۱۰۷۱۹ is relatedremarkably to different cancer risks.Materials and Methods: We performed a case-control examination of ۱۰۰ breast cancer casesand ۱۰۰ healthy controls to evaluate the association between rs۱۰۷۱۹ in DROSHA and breastcancer risk. Genomic DNA was extracted from blood samples. ARMS PCR was performed fordetection of the rs۱۰۷۱۹ by designed primers.Results: Statistical analysis showed that rs۱۰۷۱۹ polymorphism located in ۳’ untranslated region(UTR) of DROSHA gene was related to the enhanced risk of breast cancer. Additionally, thisnucleotide substitution may destroy binding site of has-miR-۶۶۴a-۳p and has-miR-۱۲۹۸-۵pwhich can cause high DROSHA protein expression in breast cancer cells.Conclusion: Altogether, our results indicated that presence of DROSHA rs۱۰۷۱۹ polymorphismand high expression of DROSHA protein could be proper tumor markers in patients with breastcancer.

کلیدواژه ها:

Breast cancer ، DROSHA ، rs۱۰۷۱۹ ، ARMS PCR ، Tumor markers