Evaluation of miR-۷۴۴ expression pattern in the blood of non-small cell lung cancer patients compared with normal individuals

Backgrounds: MicroRNAs (miRNAs) are a group of small non-coding RNAs, which arereported to play crucial roles in repressing downstream targets expressions. Therefore, theirchanges in expression are assumed to be an important biological marker for diagnosing andprognosing different types of illnesses, including non-small cell lung cancer.Materials and Methods: In this study, blood samples were collected from ۳۰ normal and ۳۰non-small cell lung cancer (NSCLC) patients. After the plasmas separation from blood samples,RNAs were extracted and cDNAs for the miR-۷۴۴ (miRNA of interest) and miR-۱۶ (referencemiRNA) were synthesized. The quantitative PCR were performed to compare the expressionlevel.Results: The results have shown a significant change in miR-۷۴۴ level in the plasma of NSCLCpatients compared with normal individuals.Conclusion: MiR-۷۴۴ could be considered as biomarker for diagnostic and therapeutic strategiesfor NSCLC.

کلیدواژه ها:

MicroRNAs ، Non-small cell lung cancer ، miR-۷۴۴ ، Quantitative PCR ، Expression level ، Biomarker