Investigation of the role of LncRNA TTTY۱۴ in colorectal cancer using Real-time PCR

Backgrounds: Colorectal cancer is the ۲nd leading cause of death in humans because of cancer.Molecular tools have been emerging as the potential biomarkers. Long non-coding RNA(LncRNAs) have shown promising capabilities to be used in clinics. The aim of this study was toinvestigate the expression of TTTY۱۴ (Testis-Specific Transcript, Y-Linked ۱۴) in colorectalcancer. This LncRNA was selected based on bioinformatics data and because of there has beenno research report on its expression in tissue samples of CRC at present.Materials and Methods: qPCR was used to measure the expression level of TTTY۱۴. For thispurpose, the colorectal tumors along with the corresponding adjacent normal tissues werecollected from thirty patients attending Milad Hospital, Isfahan, Iran. All the samples werecollected in accordance with the guidelines issued by the Ethics Committee of Isfahan Universityof Medical Sciences (approval number: ۶۳۰۷۶۰۳), regarding the ۶۴th World Medical AssociationGeneral Assembly of Helsinki declaration amended in October ۲۰۱۳. After total RNA extractionfrom samples and cDNA synthesis, Real-time PCR was used to measure the TTTY۱۴expression.Results: The qPCR results showed the expression level of TTTY۱۴ was down-regulated in thirtypaired colorectal cancer specimens (p value = ۰. ۰۰۸۵).Conclusion: Our comprehensive approach suggested that TTTY۱۴ could be an important downregulatedLncRNA in colorectal cancer. This is highly recommended to profile TTTY۱۴ inbigger colorectal cancer cohorts to see whether the aberrant expression could have clinicalrelevance.

کلیدواژه ها:

Long non-coding RNA ، Colorectal cancer ، TTTY۱۴ ، Real-time PCR