Evaluation of miR-۲۰۰a expression pattern in the blood of non-small cell lung cancer patients compared with normal individuals

Backgrounds: MicroRNAs (miRNA) are part of small noncoding RNAs family and one miRNAmay regulate many genes as its targets, while one gene may be targeted by many miRNAs. InNSCLC (non-small cell lung cancer), a significant number of abnormally expressed miRNAshave been discovered. MiR-۲۰۰a may have an important connection with apoptosis and XIAPgene in NSCLC. However, the role of miR-۲۰۰a in NSCLC is unknown.Materials and Methods: Total ۳۰ blood samples of new case NSCLC patients and ۳۰ bloodsamples of normal individuals were acquired from the Dr. Masih Daneshvari Hospital. Theplasma samples were separated from whole blood and RNAs were extracted. After the cDNAssynthesis of interest (miR-۲۰۰a) and reference (miR-۱۶) miRNAs, the quantitative PCR wereperformed to estimate the expression level.Results: The results have shown an impressive change in miR-۲۰۰a level in the plasma ofNSCLC patients compared with normal individuals.Conclusion: There is a significant correspondence between miR-۲۰۰a expression pattern andNSCLC.

کلیدواژه ها:

miRNA ، miR-۲۰۰a ، NSCLC ، Quantitative PCR ، Expression pattern