Study of Silibinin effect on PTEN in human breast cancer cell line

Backgrounds: Breast cancer is the most commonly diagnosed cancer amongst womenworldwide. Amongst several naturally-occurring flavonoids, silymarin drawn out from milkthistle plant (scientifically named Silybum marianum) has positively contributed to treatmentcancer. The PTEN gene acts as a tumor suppressor found in almost every tissue of the body. Lossor alteration of PTEN gene/protein level is often observed in various human cancers. This studyaims to evaluate the cytotoxicity of silibinin on PTEN gene in T۴۷D cell line.Materials and Methods: T۴۷D (human epithelial breast cancer) cell line was purchased fromNational Cell Bank, Tehran, Iran. T۴۷D was cultured in DMEM/F۱۲ media and treated withdifferent concentrations of silibinin (۵۰-۲۵۰ μg/mL) for ۲۴, ۴۸ and ۷۲ hours. The cytotoxic effectof silibinin on T۴۷D viability was determined using Methyl-Thiazolyl-Tetrazolium (MTT) assayby IC۵۰ determination. To assess the alterations of PTEN transcriptions, the real-time PCRreactions were performed using the Power SYBR-Green PCR Master Mix according to themanufacturer's protocol. The relative gene expression levels were calculated using the ۲−ΔΔCTmethod.Results: The IC۵۰ values for silibinin in T۴۷D Cell Line at ۴۸ hours were obtained ۱۴۴.۶μg/mL. To extracted cell total RNA, T۴۷D was seeded and incubated with IC۵۰ concentration ofSB for ۴۸h.Conclusion: mRNA expression levels demonstrate that SB significantly increased PTENexpression in T۴۷D cell line, as compared to control groups.

کلیدواژه ها:

Breast cancer ، T۴۷D ، Silibinin ، PTEN ، RT-PCR