A study of associations between DROSHA (rs۱۰۷۱۹), DICER (rs۳۷۴۲۳۳۰), RAN (rs۱۴۰۳۵) and XPO۵ (rs۱۱۰۷۷) polymorphic variants and recurrent pregnancy loss in Southeast Iranian women

Backgrounds: DROSHA, DICER, RAN and XPO۵ are factors which involved in microRNAbiogenesis and have a potential physiological role in placental development. Geneticpolymorphisms in these genes may affect the reproductive-related molecular pathways, therebypredisposing pregnant women to recurrent pregnancy loss (RPL). The aim was to investigatefour single nucleotide polymorphisms (SNPs) of DROSHA (rs۱۰۷۱۹), DICER (rs۳۷۴۲۳۳۰),RAN (rs۱۴۰۳۵) and XPO۵ (rs۱۱۰۷۷) genes in southeast Iranian women with RPL.Materials and Methods: In this study, we recruited ۱۰۰ proven RPL women (mean age ۳۲.۲۷ ±۴.۴۳ years)and ۱۰۰ control women (mean age ۳۱.۶۸ ± ۵.۲۳ years) with normal pregnancy history fromsoutheast Iranian population. Genomic DNA from whole blood was extracted and thepolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)technique was used for the selected polymorphisms.Results: A significant difference was found in the C-allele and CC genotype frequency ofDROSHA rs۱۰۷۱۹ polymorphism in RPL group compared to healthy subjects (p-value<۰.۰۵before and after adjustment). Multinomial regression analysis showed that an association ofrs۱۰۷۱۹ with risk of RPL in the recessive model after adjustment (p-value = ۰.۰۰۹). Regardingthe RAN rs۱۴۰۳۵ polymorphism, the prevalence of T-allele compared to C-allele wassignificantly different between two groups (p-value<۰.۰۰۱ before and after adjustment). As well,the CT and TT genotypes of rs۱۴۰۳۵ were associated with RPL. The genetic polymorphisms ofDICER rs۳۷۴۲۳۳ and XPO۵ rs۱۱۰۷۷ reflected no association with RPL.Conclusion: The results of this study revealed that the DROSHA rs۱۰۷۱۹ and RAN rs۱۴۰۳۵gene polymorphisms might serve as predisposing factor for RPL in Iranian women.

کلیدواژه ها:

Recurrent abortion ، DROSHA ، DICER ، RAN ، XPO۵