Evaluation of apoptosis induction in HepG۲ cell line by inhibition of miR-۴۲۷۰

Backgrounds: Hepatocellular carcinoma (HCC) is the most common type of liver cancer and avery common disease worldwide. Therefore, the discovery of new treatments in HCC isessential. MicroRNAs are small non-coding RNAs that play a role in post-transcriptional genesuppression and play a key role in regulating the cell cycle, including apoptosis. Apoptosis orprogrammed cell death has been shown to be most effective in inhibiting cancer growth. One ofthe distinctive biochemical features of apoptosis is DNA degradation by caspase-activatedDNase which makes DNA fragments ۱۸۰-۲۰۰ bp in length and are known as DNA ladders.Materials and Methods: For this purpose, HepG۲ cells were cultured in RPMI ۱۶۴۰ Mediumwith ۱۰% FBS and antibiotics at ۳۷°C. Then MTT assay was performed to determine the cellviability and the appropriate concentrations of the specific hsa-miR-۴۲۷۰ inhibitor for DNAladdering assay. Finally, DNA was extracted from hsa-miR-۴۲۷۰ inhibitor at concentrations ۲۰,۴۰ and ۸۰ nM and were electrophoresis onto the agarose gel to investigate DNA laddering andapoptosis.Results: The results showed treatment of HepG۲ cells with the hsa-miR-۴۲۷۰ inhibitor at ۴۰ and۸۰ nM concentrations induced laddering pattern of DNA and apoptosis compared to ۲۰ nM ofhsa-miR-۴۲۷۰ inhibitor and untreated HepG۲ cells.Conclusion: Inhibition of miR-۴۲۷۰ (known as an oncomiR) induced apoptosis in the HepG۲cell line and inhibited the growth of HepG۲ cells.

کلیدواژه ها:

Liver cancer ، Apoptosis ، has-miR۴۲۷۰ ، HepG۲ cell line ، HCC