The influences of chicken embryo extract on the fate of epidermal neural crest stem cells

Backgrounds: Epidermal neural crest stem cells (EPI-NCSCs) in the bulge of hair follicles are apromising source for cell-replacement therapies in neurodegenerative disease, so that isimportant to know how to increase their efficiency and effectiveness. The factor that can affectthe proliferation, morphology and gene expression of these cells is their culture medium. Theaim of this study is to observe the effect of different culture media on the proliferation,morphology and gene expression in these cells.Materials and Methods: In this study, the highly pure population of EPI-NCSCs was obtainedfrom the bulge of mouse hair follicle. Cells were implanted in four different culture media. Aftereleven days, first passage was done and when the flasks were full, the second passage was done.On the ۲۱st day, the cells were transferred to ۶-well and ۱۲-well plates to extract RNA andassess cellular morphology, respectively.Results: The results showed that the presence of chicken embryo extract increased the migrationof stem cells from the bulge of hair follicle and increased cell proliferation. Also, this extractsignificantly affected the morphology of the treated cells. In addition, evaluation of Nestin,Sox۱۰, GFAP and β-Tubulin genes in this study showed that this extract can also alter geneexpression.Conclusion: The obtained data showed the effect of ۱۱-day-old chick embryo extract in theculture medium on the migration, proliferation and morphology of epidermal neural crest stemcells.

کلیدواژه ها:

Chicken embryo extract ، Culture medium ، Epidermal neural crest stem cells ، Stem cells