Evaluation of VEGFA mRNA as non-invasive diagnostic biomarker in endometriosis

Backgrounds: In endometriosis, a prevalent woman chronic disorder, the endometrial tissue ispresent outside the uterine cavity. It disrupts the quality of women's life due to complicationsincluding pelvic pain and infertility. The delayed diagnosis by the invasive disease definitivediagnosis, laparoscopic surgery, necessitates introducing non-invasive diagnostic biomarkers.Angiogenesis is one of the essential processes involved in endometriosis pathogenesis. Thus, inthis study first, the VEGFA gene expression as angiogenesis's key factor is evaluated in aberrantendometrial tissue by bioinformatics analysis. Then the mRNA of VEGFA is measured inplasma of endometriotic women compared with normal women.Materials and Methods: The VEGFA gene expression in ectopic tissue of endometriosis andnormal endometrial tissue was compared using the GEO database and GEO۲R analysis. Then,the gene mRNA was analyzed in ۲۴ plasma samples of endometriotic women and compared withthe equivalent number of control women by the qRT-PCR.Results: In the GEO database, analyzing two datasets (GSE۲۵۶۲۸ and GSE۱۲۰۱۰۳) usingGEO۲R and P-value less than ۰.۰۵ and logFC more than ۰ showed a significantly increasedVEGFA gene expression in ectopic endometriosis tissue compared to normal endometrial tissue(with ۲۶ samples of ectopic endometriosis vs. ۲۴ normal endometria). However, qRT-PCRresults revealed no significant increase in VEGFA mRNA expression in the case group plasmaversus the control.Conclusion: The mismatch of VEGFA gene expression profile in endometrial tissue and plasmaof endometriotic women suggests no potential of this gene as a non-invasive diagnosticbiomarker for endometriosis despite its importance in the pathogenesis of endometriosis.

کلیدواژه ها:

VEGFA ، Non-invasive diagnosis ، Endometriosis ، GEO ، qRT-PCR