Comprehensive bioinformatics analysis of mir-۶۱۶ in triple negative breast cancer
محل انتشار: کنفرانس بین المللی ژنتیک و ژنومیکس انسانی
سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 130
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شناسه ملی سند علمی:
CHGGE01_121
تاریخ نمایه سازی: 13 مهر 1401
چکیده مقاله:
Backgrounds: MicroRNAs (miRNAs) are endogenous, small non-coding RNAs with functionin the regulation of gene expression. The expression of miRNAs is dysregulated in numeroushuman cancers through various mechanisms. Mir-۶۱۶ is newly confirmed to be a cancer-relatedmiRNA. miR-۶۱۶ was proved as up-regulated miRNA in triple-negative breast cancer (TNBC).It was known that TNBC patients with up-regulated miR-۶۱۶ had a poor prognosis. The aim ofthis study is to carefully examine the molecular mechanism of mir-۶۱۶ in TNBC pathogenesis.Materials and Methods: Briefly, the mRNA dataset (GSE۳۸۹۵۹) was retrieved from GEO toidentify the differentially expressed genes (DEGs). The target genes of hsa-miR-۶۱۶ werepredicted using miRWALK and TargetScan. Overlapping genes between DEGs and has-miR-۶۱۶ targets were subjected to protein-protein interaction network (PPIN) construction viaSTRINGdb R package. Down-regulated genes are merely filtered out due to reciprocalregulation. Finally, GO and KEGG enrichment analyses were used.Results: A total of ۱۱۶ overlapping genes related to TNBC and miR-۶۱۶ were found. Only ۳۱genes, out of the total ۱۱۶ genes, were selected which have reciprocal regulation with mir-۶۱۶expression. Genes were enriched for pathways in cancer including estrogen signaling pathway,neurotrophin signaling pathway, JAK-STAT signaling pathway, and PI۳K-Akt signalingpathway. Additionally, GO enrichment analysis indicated steroid hormone and protein kinase Bsignaling pathway, transcription regulation, and phosphatidylinositol ۳-kinase complex.Conclusion: In conclusion, we investigated the miR-۶۱۶ molecular mechanism in TNBC andprovided a comprehensive view of the underlying mechanisms. These can be further studied indetail in order to find new targeted therapies for TNBC.
کلیدواژه ها:
Triple negative breast cancer ، MicroRNAs ، Functional annotation ، Signaling pathways ، Differentially expressed gene
نویسندگان
Mohammadali Izadpanah Kazemi
Department of Biology, Faculty of basic sciences, Islamic Azad University, Mashhad, Iran
Aria Jahanimoghadam
Department of Bioinformatics, Biozentrum, University of Würzburg, Würzburg, Germany
Zahra Mahmoodkhani
Department of Biophysics, Faculty of Science, Tarbiat Modarres University, Tehran, Iran
Negin Ahmadi Jazi
Department of Zoology, Faculty of Biological Science, Shahid Beheshti University, Tehran, Iran
Reza Gheitasi
Institute for Infectious Diseases and Infection Control, Jena University Hospital, Jena, Germany