Antibiotic Susceptibility and Prevalence of AdhesionGenes in Streptococcus pneumoniae IsolatesDetected in Carrier Children in Tehran
سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 174
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شناسه ملی سند علمی:
SETBCONF02_156
تاریخ نمایه سازی: 21 شهریور 1401
چکیده مقاله:
Background:Pharyngeal carriers are the source and transitional vectors of invasive diseases. Attachment is the first step in pathogenicity. Strains of Streptococcus as normal flora can cause diseases in certain circumstances. Adhesion proteins of these bacteriaplay a fundamental role in the attachment and colonization.Objectives:In the present study, ۵ genes encoding surface proteins namely phtD, pspC, phtE, lytA, and rrgA were evaluated in Streptococcus pneumoniae isolates collected from ۴ main care centers and the children’s Medical center in Tehran.Methods:Three hundred and eight nasopharyngeal swab specimens were collected from children under ۶ years. The identification of S. pneumoniae isolates was performed using biochemical tests confirmed by PCR for the presence of cpsA gene. The existence of phtD, phtE, pspC, lytA, and rrgA genes was studied by PCR amplification assays.Results:From۳۰۸ nasopharyngeal swabs, ۱۰۲ isolates of S. pneumoniae wereconfirmedby identification tests. Amongthese isolates, ۸۷ (۸۵.۲%), ۵۴ (۵۲.۹%), ۵۱ (۵۰%), ۴۳ (۴۲.۱%), and ۳۱ (۳۰.۳%) were positive for lytA, rrgA, phtE, pspC, and phtD genes, respectively.Conclusions:Our study showed that cpsA of S. pneumoniae is one of the major characteristic genetic markers for diagnostic purposes. Among five adhesion genes, lytA was the most frequent one and the strains with a combination of rrgA and lytA genes were predominant. These findings could be very useful in designing further studies on vaccines against S. pneumoniae in our country.
کلیدواژه ها:
نویسندگان
Sara Abdollahi
Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
Seyed Mohammad Ghorbani Dazmiri
Faculty of Biological Sciences, Sari Branch, Islamic Azad University, Sari, Iran
Masoumeh MirzaeiChegeni
Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran
Shila Fallahpour
Department of Biology, Biological Science Faculty, Islamic Azad University of Falavarjan, Isfahan,Iran
Shaghayegh Norouzidehmajnooni
Department of Biology, Islamic Azad University of Yazd, Yazd, Iran
Zahra Abad
Department of biological sciences,cellular and molecular biology research center, North Tehran branch, Islamic Azad university,Tehran, Iran