Investigation of the endometrial receptivity status in experimental hypothyroid-induced female rats

Objective(s): This study aimed to investigate hypothyroidism’s effects on endometrial receptivity, creating an experimental hypothyroidism model in female rats. Materials and Methods: To induce hypothyroidism in rats of Hipotiroid-ER and Treatment-ER groups, ۰.۰۵% ۶-propyl-۲-thiouracil was freshly added to their drinking water for ۸ weeks and then the endometrial receptivity model was applied and sacrificed on the fifth day. In the Treatment-ER group, after sc-administration of ۰.۸ µg/۱۰۰ g L-thyroxine for ۱۰ days, the endometrial receptivity model was applied to the rats and sacrificed on the fifth day. Results: In the histopathological evaluation, epithelial degeneration, vacuolization, enlargement of the uterine glands, and morphological disorders were observed in the endometrial layer of the Hypothyroid-ER group. However, these pathologies were significantly alleviated in the Treatment-ER group. Integrin β۳, integrin αvβ۳, LIF, and HOXA۱۰ immune reaction intensities were high in the Control-ER and Treatment-ER groups, while in the Hypothyroid-ER group, integrin β۳, integrin αvβ۳, LIF, and HOXA۱۰ immunoreactivity intensities were low. Also, while MUC۱ immunoreactivity was high in the Hypothyroid-ER group, it was low in the other groups. In biochemical analysis, a significant increase in the TSH and progesterone levels and a significant decrease in the FT۴, E۲, FSH, and LH levels in the Hypothyroid-ER group compared with the Control-ER group were observed. Also, all hormone levels were significantly ameliorated in the rats of the Treatment-ER group compared with the Hypothyroid-ER group. Conclusion: The results obtained showed that hypothyroidism had a significant effect on endometrial receptivity—the histopathological and biochemical changes caused by hypothyroidism in the experimental rat model were ameliorated with L-thyroxine treatment.

کلیدواژه ها:

Endometrium ، Hypothyroidism ، Infertility ، Propylthiouracil ، thyroxine