Antibacterial Activity of Ethanolic Extract of Matricaria chamomilla, Malva cylvestris, and Capsella bursa-pastoris against Multidrug-Resistant Pseudomonas aeruginosa Strains

Background: This study aimed to determine antibacterial activity of ethanolic extract of Matricaria chamomilla(chamomile), Malva sylvestris, and Capsella bursa-pastoris against multidrug-resistant (MDR) clinical isolates ofPseudomonas aeruginosa.Methods: The plants were collected from Ziarat village of Gorgan, Iran in April ۲۰۱۹. The required parts ofthe plants were separated and completely dried in the shade. After grinding, extraction was performed bymaceration method. The extract was dried at ۳۷°C for ۲۴ hours. To obtain a concentration of ۵۰ mg/mL ofeach extract, ۵۰۰ mg of the dried plant extract was dissolved in ۱۰ mL ۵% dimethyl sulfoxide and sterilizedby filtration through a ۰.۴۵ μm membrane filter. For the antibacterial assay, agar well diffusion and brothmicrodilution methods were used.Results: Based on the results, ethanolic extracts of M. sylvestris and Capsella bursa-pastoris did not show anyantibacterial activity against MDR P. aeruginosa isolates in both antibacterial assays. No inhibitory effect wasobserved for ethanolic extract of chamomile against P. aeruginosa isolates in agar well diffusion method as well.In broth microdilution method, the extract of chamomile leaves showed inhibitory effect and the minimuminhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined as ۱۲.۵ and۲۵ mg/mL, respectively.Conclusions: In this study, the extract of ethanolic chamomile leaves showed antibacterial activity against theMDR P. aeruginosa isolates. Thus, it can be used in the production of antibacterial agents, and it is a good optionfor protection against pathogenic microorganisms, as well as P. aeruginosa.

کلیدواژه ها:

Antibacterial activity ، Matricaria chamomilla ، Malva sylvestris ، Capsella bursa-pastoris ، Pseudomonasaeruginosa