Effect of ۵۰-Hz Magnetic Fields on Argyrophilic Nucleolar Organizer Regions in Bone Marrow Cells
محل انتشار: مجله تحقیقات دارویی ابن سینا، دوره: 2، شماره: 1
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 255
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شناسه ملی سند علمی:
JR_AJPR-2-1_004
تاریخ نمایه سازی: 30 مرداد 1401
چکیده مقاله:
Background: Exposure to an increasing amount of extremely low-frequency magnetic fields (MFs) causessome adverse effects. Considering a direct association between the sizes and numbers of argyrophilicnucleolar organizer regions (AgNORs) and cell proliferation, this study was conducted to evaluate theeffect of ۵۰-Hz MFs on the AgNORs of bone marrow (BM) cells.Methods: Eighty male rats were randomly divided into four exposed groups and one control group. Theexposed groups received ۵۰-Hz MFs with magnetic flux densities of ۱, ۱۰۰, ۵۰۰, and ۲۰۰۰ μT two hoursa day for two months. BM cells were aspirated from sacrificed rats’ femoral bones, smeared on glassslides, and then stained with silver nitrate for AgNORs. The area (AA), length (AL), and number (AN) ofAgNORs were calculated by a microscope equipped with a camera and Scion Image software in ۱۰۰ BMcells of each rat. The mean of AA, AL, and AN was computed for each group.Results: AA, AL, and AN significantly reduced in the ۱ μT group compared with ۲۰۰۰ μT and controlgroups. Eventually, there was a nonlinear relationship between the effect of ۵۰-Hz MFs and magneticflux densities.Conclusion: Overall, ۵۰-Hz MFs with a magnetic flux density of ۱ μT reduced AgNORs in BM cells.However, ۱۰۰, ۵۰۰, and ۲۰۰۰ μT did not affect AgNORs. Therefore, ۵۰-Hz MFs with low density maysuppress BM cell proliferation.
کلیدواژه ها:
نویسندگان
Mohammad Rafiee
Department of Medical Laboratory Sciences, School of Paramedicine, Hamadan University of Medical Sciences,Hamadan, Iran
Mohammad Abbasi
Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Mohammad Yousef Alikhani
Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Safoora Nikzad
Department of Medical Physics, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Alireza Zamani
Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran