Foliar application of yeast extract and salicylic acid affect chemical composition and content of lemon balm (Melissa officinalis L.) essential oil

The present study investigated the effect of ۱۴ treatments consisting yeast extract (YE) (۰, ۰.۵, ۱.۰, ۱.۵, ۲ g/l), salicylic acid (SA) (۰, ۴۰, ۸۰, ۱۶۰, ۳۲۰ mg/l) and YE (۱ and ۱.۵ g/l) in combination with SA (۸۰ and ۱۶۰ mg/l) foliar application on essential oil content and constituents of lemon balm (Melissa officinalis L.). The experiment was conducted in a completely randomized design with three replications under greenhouse conditions. Essential oils analyzed by GC/MS and a total of ۳۹ compounds were identified that the major constituents were citronellol, trans-carveol, γ-۳-carene, linalool, citral and carvacrol acetate, respectively (۴۲.۸ to ۴۸.۰% in total). Citronellol was the main constituent of essential oils with ۱۱.۰۵%. SA and YE significantly altered the amount of ۲۳ constituents of lemon balm essential oil (P˂۰.۰۱). The highest citronellol, linalool and citral (۱۴.۵۰, ۷.۹ and ۸%, respectively) production was obtained at ۱.۵ g/l YE+۱۶۰ mg/l SA treated plants that was ۱۰۳, ۸۸ and ۲۰۳% higher than control plants, respectively. The highest essential oil content (۰.۳۳۶% v/w) that was ۴۹% higher than control was achieved by ۱.۰ and ۱.۵g/l YE+۱۶۰ mg/l SA treatments. The principal component analysis (PCA) and heatmap indicated that the content of compounds varied with different treatment and also revealed a clear separation between control and treatment groups. The results suggested that SA, YE and SA in combination with YE has considerable ability to stimulate the production of major constituent such as citronellol, citral, and linalool in the lemon balm.

کلیدواژه ها:

Citral ، citronellol ، Elicitor ، Lamiaceae ، Medicinal plant