Testing Two Chromosome Doubling Agents for in vitro Tetraploid Induction on Ginger Lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig

The genus Hedychium consists of about ۸۰ species, which are identified by attractive foliage as well as various dramatic and fragrant flowers. Some species are cultivated for edible flowers, and others for medicinal or industrial properties (He ۲۰۰۰; Gopanraj et al. ۲۰۰۵; Gao et al. ۲۰۰۸). Most species of Hedychium are native to Central and Southeast Asia, with high concentrations in southern China and the Himalayas (Hayes et al. ۲۰۰۹). Induced polyploidy is a valuable tool for breeding that gives some advantages for horticultural, pharmaceutical and agricultural improvement of plants. The in vitro induction of polyploids with growth regulators such as colchicine or oryzalin has been published in many plant species. Increased ploidy level resulted in increased flower size in Gerbera jamesonii Bolus cv. Sciella (Gantait et al. ۲۰۱۱) and intensified flower colours in cyclamen (Takarnura and Miyajima ۱۹۹۶). Establishing optimum in vitro cultural conditions is indispensable for the efficient in vitro induction of polyploid plants. Chromosome doubling of diploid in ornamental plants is key to increased organ size, prolonged flowering period or increased resistance to abiotic stresses, diseases, and pests (Dhooghe et al. ۲۰۱۱). The present study examined the effect of different reagents with various concentrations on polyploidy induction of two ornamental accessions of Hedychium for horticultural purposes with possible different morphological and biological characteristics.

کلیدواژه ها:

Hedychium ، Colchicine ، Oryzalin ، Somatic chromosome doubling